The State Emergency Relief Fund was established to provide assistance to those impacted by a disaster and 100% of donations will be used to help people and businesses re-establish themselves after the South Australian bushfires.
The Olympic Dam mine at Roxby Downs is one of the state's major employers.
"The bushfires have had a considerable impact on hundreds of lives and for families who have lost their homes, it is devastating," Olympic Dam asset president Darryl Cazzubbo said.
"We hope this donation will provide some support for those experiencing difficulties during this time."
BHP Billiton Sustainable Communities will also donate $2 for every $1 donated by an employee to relief efforts through the BHP Billiton Matched Giving Program.
BHP Billiton Sustainable Communities is a charity established by BHP Billiton as part of its community investment program.